Journal:

Article Title: Induction of Specific Cytotoxic Lymphocytes in Mice Vaccinated with Brucella abortus RB51

doi: 10.1128/IAI.69.9.5502-5508.2001

Figure Lengend Snippet: Different roles of CD4+ and CD8+ T cells in the cytotoxic lysis and release of IFN-γ. The two T-cell populations were isolated by the MACS magnetic kit and showed more than 92% purity by flow cytometry. The individual T-cell populations were cocultured at various concentrations with either strain RB51-infected or normal J774.A1 cells. The cytotoxic activity (A) and the amount of IFN-γ released into the supernatants (B) were measured. The data are means for triplicate estimations, and standard deviations did not exceed 20% of the means. Cocultures of CD4+ T cells with noninfected target macrophages (solid squares) and RB51-pulsed target macrophages (solid triangles) and CD8+ T cells with noninfected target cells (solid diamonds) and RB51-pulsed target cells (solid circles) were tested.

Article Snippet: Briefly, live T cells isolated by Histopaque column purification were incubated with MACS magnetic MicroBeads to which monoclonal antibodies against CD4 molecule (clone GK1.5; isotype, rat IgG2b) or CD8 molecule (clone 53-6.7; isotype, rat IgG2a) had been coupled (Miltenyi Biotec, Auburn, Calif.) at the concentration of 10 μl of MicroBeads per 10 7 total cells for 15 min in a refrigerator at 4°C (see Fig. ).

Techniques: Lysis, Isolation, Flow Cytometry, Infection, Activity Assay

Journal:

Article Title: Induction of Specific Cytotoxic Lymphocytes in Mice Vaccinated with Brucella abortus RB51

doi: 10.1128/IAI.69.9.5502-5508.2001

Figure Lengend Snippet: Specific phenotype analysis of effector cells by flow cytometry a

Article Snippet: Briefly, live T cells isolated by Histopaque column purification were incubated with MACS magnetic MicroBeads to which monoclonal antibodies against CD4 molecule (clone GK1.5; isotype, rat IgG2b) or CD8 molecule (clone 53-6.7; isotype, rat IgG2a) had been coupled (Miltenyi Biotec, Auburn, Calif.) at the concentration of 10 μl of MicroBeads per 10 7 total cells for 15 min in a refrigerator at 4°C (see Fig. ).

Techniques: Flow Cytometry

Journal: Nature Communications

Article Title: Fasting mimicking diet in mice delays cancer growth and reduces immunotherapy-associated cardiovascular and systemic side effects

doi: 10.1038/s41467-023-41066-3

Figure Lengend Snippet:

Article Snippet: Anti-mouse CD4, VioBright FITC (REA604) , Miltenyi Biotec , 130-118-692.

Techniques: In Vivo

Journal: Cell reports

Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype

doi: 10.1016/j.celrep.2022.111462

Figure Lengend Snippet:

Article Snippet: Anti-Mouse CD4 (RM4-5)-145Nd , Fluidigm , Cat# 3145002B; RRID:AB_2687832.

Techniques: Control, Recombinant, Liposomes, Labeling, Quantitation Assay, Staining, RNA Sequencing, Mass Spectrometry, Knock-Out, Software

Journal: Cell Reports Medicine

Article Title: Cathepsin-D-mediated MHC class I degradation contributes to immune evasion in colorectal cancer

doi: 10.1016/j.xcrm.2025.102534

Figure Lengend Snippet: Elevated CTSD expression correlates with reduced immune cell infiltration in CRC (A) Venn diagram shows overlapping of upregulated genes from RNA-seq data comparing MSS versus MSI-H and focus on upregulated genes associated with immunotherapy response in MSS CRC. Based on log 2FC > 1 and p < 0.05. (B) Representative immunohistochemical staining images of CTSD in CRC tissues. Scale bars, 100 μm. (C) Histogram showing the overall response rate in patients with low and high CTSD expression levels in the refractory MSS CRC cohort. (D) Representative computed tomography (CT) images of patients with lung metastasis of CRC before and after anti-PD-1 mAb (PD-1) treatment. Red circles indicate the location of lung metastases. (E) Kaplan-Meier analysis of PFS comparing patients with low and high CTSD expression in the refractory MSS CRC cohort. (F and G) CTSD expression in CRC tissues stratified by invasion (F) and stage (G). (H) Overall survival curves were generated based on the protein levels of CTSD in CRC. (I–L) Multiplex immunofluorescence analysis of immune cell infiltration intensity between low and high CTSD expression groups. Tumor samples were stained with specific antibodies to CTSD in the red fluorescence channel, CD8 in celeste, CD163 in green, CD4 in purple, and cell nuclei in blue by DAPI staining (I). Scale bars, 50 μm. Statistics of CD8 (J), CD4 (K), and CD163 (L) cells based on CTSD expression. (M and N) Kaplan-Meier survival curves for OS and PFS analysis of patients based on relative expression of CTSD in the Kaplan-Meier plotter immunotherapy cohort showing pan-cancer samples with anti-PD-1 (pembrolizumab) or anti-CTLA4 (ipilimumab) treatment. Data are means ± SEM. p values were determined by log rank test (E, H, M, and N), unpaired Student’s t test (F, G, and J–L), and Fisher’s exact test (C).

Article Snippet: InVivoMAb anti-mouse CD4 , Bio X Cell , Cat# BE0003-1; RRID: AB_1107636.

Techniques: Expressing, RNA Sequencing, Immunohistochemical staining, Staining, Computed Tomography, Generated, Multiplex Assay, Immunofluorescence, Fluorescence

Journal: Cell Reports Medicine

Article Title: Cathepsin-D-mediated MHC class I degradation contributes to immune evasion in colorectal cancer

doi: 10.1016/j.xcrm.2025.102534

Figure Lengend Snippet: Suppression of CTSD boosts cytotoxic T cell activation and enhances anti-tumor immunotherapeutic responses (A) Primary tumors from mice were harvested for flow cytometry to determine the percentages of GZMB + and IFN-γ + cells among CD8 + T cells and T-bet + and Foxp3 + among CD4 + T cell ( n = 6). (B and C) Tumor volume, representative tumor images, and tumor weight from CTSD knockdown CT26 tumor cells in BALB/c mice depleted of CD8 + /CD4 + T cells. s.c represents subcutaneous injection. (D) Immunofluorescent staining of CD8 in CT26 tumor and quantification of CD8 signals ( n = 6). Scale bars, 50 μm. (E) Primary tumors from mice were harvested for flow cytometry to determine the percentages of Ki67 + cells among CD8 + T cells ( n = 6). (F and G) BALB/c mice bearing subcutaneous CT26 tumor cell expressing shCTSD, received IgG, or antiPD-1 antibody therapy. Tumor volumes (F) and Kaplan-Meier survival curves (G) on the indicated days after inoculation with CT26 cells ( n = 6). s.c represents subcutaneous injection. (H) Immunofluorescent staining of CD8 in CT26 tumors from (F); quantification of CD8 signals ( n = 6). Scale bars, 50 μm. (I) Primary tumors from mice were harvested for flow cytometry to determine the percentages of GZMB + and IFN-γ + cells among CD8 + T cells ( n = 6). Data are means ± SEM. p values were calculated by two-tailed Student’s t test (A, C, D, and E), one-way ANOVA (H and I), two-way ANOVA for tumor volumes (F), and log rank test for Kaplan-Meier survival curves (G). n.s represents no significance.

Article Snippet: InVivoMAb anti-mouse CD4 , Bio X Cell , Cat# BE0003-1; RRID: AB_1107636.

Techniques: Activation Assay, Flow Cytometry, Knockdown, Injection, Staining, Expressing, Two Tailed Test

Journal: Cancer Cell

Article Title: Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity

doi: 10.1016/j.ccell.2022.08.014

Figure Lengend Snippet: IM + anti-VEGF prolongs survival of GBM mice and is immunostimulatory (A) Schematic of the long-term therapeutic trials in the lentiviral-induced mouse model of glioma. (B) Representative images of H&E-stained tissue sections from a tumor that developed in an end-stage LVRshp53 animal. Scale bar, 30 μm. Representative of whole-slide images of three tumors. (C) Survival of tumor-bearing LVRshp53 animals subjected to the indicated treatments. Control (Ctrl) (n = 10), anti-VEGF (n = 7), IM + anti-VEGF (n = 7), IM + anti-VEGF + CDL (n = 8). (D) Normalized bioluminescence in LVRshp53 animals treated as indicated for 2 weeks. (E) Survival of PDG animals subjected to the indicated treatments. Ctrl (n = 9), IM + anti-VEGF (n = 10). (F) Representative images of CD8 (green) and DAPI nuclear staining (blue). Scale bar, 50 μm. Image is illustrative of the analysis shown in (H). (G) High-magnification images of CD8 T cells in a Ctrl versus an IM + anti-VEGF-treated tumor. Scale bar, 50 mm (H) Quantification of CD8 T cells in LVRshp53 tumors treated as indicated for 12 days. Each dot indicates the average of 8–12 immuno-stained tumor tissue sections from one mouse. (I) Flow cytometry analysis of CD8 T cells in LVRshp53 tumors treated as indicated for 12 days. Cells were gated as CD45 + CD3 + CD8 + . Ctrl (n = 15), anti-VEGF (n = 8), IM (n = 10), IM + anti-VEGF (n = 10). (J and K) Representative images (J) and quantification (K) of CD4 T cells in whole LVRshp53 tumor tissue section. Animals were treated for 12 days. Scale bar, 50 μm. Ctrl (n = 6), anti-VEGF (n = 4), IM (n = 4), IM + anti-VEGF (n = 6). (L) Assessment of the functional contributions of CD8 and CD4 T cells to survival benefit. Ctrl (n = 6), αCD8 + αCD4 (n = 4), IM + anti-VEGF (n = 5), IM + anti-VEGF + αCD8 + αCD4 (n = 5), IM + anti-VEGF + αCD8 (n = 5). (M) Normalized bioluminescence in LVRshp53 mice treated as indicated in (L). (Para break) Data in all quantitative panels are shown as mean ± SEM. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns, no statistical significance. For survival analyses, Mantel-Cox test was performed. Other analyses by Mann-Whitney or one-way ANOVA tests.

Article Snippet: InVivo MAb anti-mouse CD4 (clone GK1.5) , BioXCell , Cat #: BE0003-1; RRID: AB_1107636.

Techniques: Staining, Control, Flow Cytometry, Functional Assay, MANN-WHITNEY

Journal: Cancer Cell

Article Title: Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity

doi: 10.1016/j.ccell.2022.08.014

Figure Lengend Snippet: CD8 and CD4 T cells are activated upon IM + anti-VEGF treatment (A) Flow cytometry analysis of effector T cells (CD62L-CD44 + ). Ctrl (n = 6), IM (n = 4), anti-VEGF (n = 7), and IM + anti-VEGF (n = 7). (B) FACS analysis of IFNγ intracellular staining in fixed and permeabilized CD8+T cells. Ctrl (n = 16), IM (n = 10), anti-VEGF (n = 12), IM + anti-VEGF (n = 12). (C and D) Flow cytometry analysis of GzB (C) and TNFα (D) intracellular staining in CD8 T cells. Ctrl (n = 15), IM (n = 10), anti-VEGF (n = 8), IM + anti-VEGF (n = 8). (E) Functional importance of IFNγ for the survival of LVRshp53 animals subjected to the indicated treatments. Ctrl (n = 9), anti-IFNγ (n = 5), IM + anti-VEGF (n = 7), IM + anti-VEGF + anti-IFNγ (n = 6). Statistical analysis by Mantel-Cox test. (F, G, and H) Flow cytometry analysis of Ki67 (F), pSTAT5 (G), and TCF1 (H) in CD8 T cells. Ctrl (n = 7), IM (n = 4), anti-VEGF (n = 9), and IM + anti-VEGF (n = 10). (I) Representative image of HIF-1α (red) and CD8 (green) in the anti-VEGF-treated tumor. Image is illustrative of the analysis performed in (J). Scale bar, 50 mm (J) Quantification of the proximity of CD8 T cells to hypoxic regions in the entire area of full sections of GBM tumors. The zones were divided into 0 μm (i.e., within the HIF-1α+ zone), >0 and <5 μm, and >5 μm separating T cells and HIF-1α+ regions. Ctrl (n = 11), IM (n = 4), anti-VEGF (n = 4), IM + anti-VEGF (n = 5). (K) Flow cytometry analysis of intracellular HIF-1α expression in fixed and permeabilized CD8 T cells. Ctrl (n = 5), IM (n = 4), anti-VEGF (n = 8), anti-VEGFR2 (n = 5). (L) Flow cytometry analysis of intracellular FOXP3 expression in CD4 T cells. Ctrl (n = 5), IM (n = 5), anti-VEGF (n = 4), IM + anti-VEGF (n = 8). (M) Flow cytometry analysis of intracellular TGFβ expression in CD4 T cells. Ctrl (n = 5), IM (n = 5), anti-VEGF (n = 4), IM + anti-VEGF (n = 7). (N and O) Flow cytometry analysis of SLAMF7 (N) and GzB (O) expression in CD4 T cells. Ctrl (n = 8), IM (n = 5), anti-VEGF (n = 5), IM + anti-VEGF (n = 7). (Para break) Data in all quantitative panels are shown as mean ± SEM. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns, no statistical significance. Statistical analysis by one-way ANOVA, unless otherwise indicated.

Article Snippet: InVivo MAb anti-mouse CD4 (clone GK1.5) , BioXCell , Cat #: BE0003-1; RRID: AB_1107636.

Techniques: Flow Cytometry, Staining, Functional Assay, Expressing

Journal: Cancer Cell

Article Title: Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity

doi: 10.1016/j.ccell.2022.08.014

Figure Lengend Snippet: Imipramine downregulates an M2-like program in TAMs (A) Western blot analysis of ARG1 and IL-10 in single tumors treated or not with IM or anti-VEGF. (B) Flow cytometry analysis of ARG1 and IL-10 expression in GBM tumors treated for 1 week. Ctrl (n = 9 tumors), IM (n = 6), anti-VEGF (n = 6), anti-VEGFR2 (n = 6), Axitinib (n = 6). Macrophages were gated as CD45 + CD11b+Ly6C-Ly6G−. (C) Expression of ARG1 and IL-10 in microglia (CD49d−) and MDMs (CD49d+) assessed by FACS in untreated tumors (n = 5). (D) Expression of MHC-II within microglia as assessed by flow cytometry. Ctrl (n = 4 tumors) and IM + anti-VEGF (n = 4). (E) Ex vivo co-cultures of tumoral CD11b cells and activated splenic CFSE-labeled CD8 or CD4 T cells. Each dot represents the average of two or three technical replicates. T cells alone (n = 4), Ctrl co-culture (n = 5), anti-VEGF (n = 3), IM (n = 4), IM + anti-VEGF (n = 4). (F) Analysis of the M2-like program in cytokine-polarized macrophages as assessed by qRT-PCR analysis of Ctrl and IM-treated M2-like BMDMs. Expression is normalized to 18S statistics by Welch’s t test. Each dot represents an individual sample. Data are representative of three independent experiments. (G) Analysis of the M1-like program in BMDMs assessed by FACS. Each dot represents an individual replicate. (H) Expression of Hrh1 mRNA normalized to 18S in ex vivo M1-and M2-polarized BMDMs, either untreated or IM treated for 24 h. Each dot represents an individual sample. Data are representative of three independent experiments. (I) mRNA expression of Hrh1 in FACS-sorted microglia or MDMs from Ctrl and IM-treated tumors. (J) mRNA expression of Arg1 , Chil3 , and Il10 in M2-polarized macrophages that were transfected with si Ctrl or two different si Hrh1 constructs. Cells were treated with 40 μm IM for 24 h. Data are representative of two independent experiments. (K) Western blot analysis of MRC1 and ARG1 expression of siRNA-transfected M2 BMDMs. Data are representative of two independent experiments. (L) CD8 and CD4 T cell proliferation during co-culture with tumoral CD11b cells isolated from untreated (n = 2) or TFP-treated tumors (n = 4). (M) mRNA expression of Hrh1 , Arg1 , and MMP2 in CD11b cells isolated from tumors treated with IM (n = 4), TFP (n = 4), or untreated Ctrl (n = 5). (N) Phagocytosis assay involving sorted microglia and MDMs from untreated or IM-treated tumors assayed with green pHrodo S. aureus bioparticles. Data presented as mean fluorescence intensity (MFI) of pHrodo/live cells. (Para break) Data in all quantitative panels are presented as mean ± SEM ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns, no statistical significance. Statistical analysis by Mann-Whitney test or one-way ANOVA, unless otherwise stated.

Article Snippet: InVivo MAb anti-mouse CD4 (clone GK1.5) , BioXCell , Cat #: BE0003-1; RRID: AB_1107636.

Techniques: Western Blot, Flow Cytometry, Expressing, Ex Vivo, Labeling, Co-Culture Assay, Quantitative RT-PCR, Transfection, Construct, Isolation, Phagocytosis Assay, Fluorescence, MANN-WHITNEY

Journal: Cancer Cell

Article Title: Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity

doi: 10.1016/j.ccell.2022.08.014

Figure Lengend Snippet: Macrophage-derived CXCR3 ligands are required for the therapeutic benefit conveyed by the combinatorial regimen of IM + anti-VEGF (A) Cxcl10 and Cxcl9 expression in bulk tumors. mRNA expression is shown relative to Gapdh . Ctrl (n = 6), anti-VEGF (n = 9), IM (n = 6), IM + anti-VEGF (n = 8). (B) Representative image of CXCL10 (magenta), F4/80 (red), and DAPI (blue) staining of LVRshp53 tumors treated with IM + anti-VEGF. Scale bar, 50 μm. Images are illustrative of five to six fields in tissue sections from three different tumors. (C) CXCL9 expression in TAMs in untreated (n = 6) or tumors treated with anti-VEGF (n = 4), IM (n = 4), or IM + anti-VEGF (n = 8) revealed by flow cytometry. (D) CXCL9 expression in MDMs and microglia, evaluated as in (C). (E) mRNA Cxcl9 and Cxcl10 expression assessed in bulk tumors treated with IM + anti-VEGF (n = 10) ± αCD49d (n = 7) to selectively deplete MDMs but not microglia. Expression is normalized to Gapdh housekeeping gene. (F) Assessing the contribution of CXCR3 function to the survival of LVRshp53 animals subjected to the indicated treatments. Treatment cohorts: αCXCR3 (n = 6), IM + anti-VEGF + αCXCR3 (n = 6), IM + anti-VEGF (n = 5). (G) Representative images of CD8 T cells aimed to assess the effects of αCXCR3. Representative of whole-slide image analysis of three tumors per treatment. Scale bar, 50 mm (H) Flow cytometry analysis of CD8 T cells in tumors subjected to indicated treatments. (I) Ex vivo co-culture of tumor-derived CD11b cells and CFSE-labeled CD8 or CD4 T cells. Myeloid cells were isolated from tumors treated with IM + anti-VEGF (n = 4), IM + anti-VEGF + αCXCR3 (n = 4), or untreated Ctrl (n = 5). Each dot represents an average of two or three technical replicates. (J) Minimal effect on IFNγ secretion by CD8 T cells in tumors treated with αCXCR3, IM + anti-VEGF, or the triple combination. (K and L) No effect of αCXCR3 on (K) GzB or (L) TNFα secretion by CD8 T cells co-treated with IM + anti-VEGF. (M) Quantification of immunostaining for HEVs in tumors treated with IM + anti-VEGF (n = 8) or IM + anti-VEGF + αCXCR3 (n = 4). The data are shown as number of HEVs per square millimeter of tumor tissue. (Para break) Data in all quantitative panels are presented as mean ± SEM. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns, no statistical significance. Statistical analysis by Mann-Whitney test or one-way ANOVA, unless otherwise stated.

Article Snippet: InVivo MAb anti-mouse CD4 (clone GK1.5) , BioXCell , Cat #: BE0003-1; RRID: AB_1107636.

Techniques: Derivative Assay, Expressing, Staining, Flow Cytometry, Ex Vivo, Co-Culture Assay, Labeling, Isolation, Immunostaining, MANN-WHITNEY

Journal: Cancer Cell

Article Title: Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity

doi: 10.1016/j.ccell.2022.08.014

Figure Lengend Snippet: PD-L1 is induced in relapsing tumors and its blockade potentiates T cell function to prolong survival benefit in GBM mice (A) FACS analysis of PD-L1 in the live cell compartment of tumors treated as indicated. Ctrl (n = 4 tumors), responding to IM + anti-VEGF (n = 4), and relapsing from IM + anti-VEGF (n = 8). Responding tumors were collected after 12 days of treatment. Relapsing tumors were collected when mice became symptomatic or when tumors started to re-grow following a stable phase. (B) Representative image of immunostaining to reveal PD-L1 (red), CD45 (green), and DAPI nuclei (blue) in relapsing tumors after IM + anti-VEGF. Scale bar, 50 μm. Assessed in four relapsing tumors, n = 8–10 fields imaged per tumor. (C) Percentage of PD-L1-positive live cells comparing the CD45 − and CD45 + compartments of n = 4 relapsing GBM tumors as revealed by flow cytometry. (D) PD-L1 expression in the CD11b− and CD11b+ compartments of CD45 + cells in n = 8 tumors assessed by flow cytometry. (E) Percentage of PD-L1-positive TAMs assessed by FACS. Ctrl (n = 4), responding tumor (n = 4), relapsing tumor (n = 8). (F) Representative immunostaining to reveal PD-L1 expression in TAMs. CD206 (magenta), PD-L1 (red), and DAPI in n = 3 relapsing tumors, 8–10 images per tumor. Scale bar, 50 μm. (G) Expression of PD-L1 in MDMs and microglia of n = 8 relapsing tumors assessed by flow cytometry. (H) MHC-II expression in microglia comparing responding (n = 4) and non-responding tumors (n = 4), assessed by flow cytometry. (I and J) Representative images (I) and quantification (J) of CD8 T cells in untreated (n = 3), responding (n = 4), and relapsing (n = 3) tumors under IM + anti-VEGF treatment. CD8 (magenta) and DAPI-stained nuclei. Scale bar, 50 μm. Each dot indicates the total number of CD8 T cells in an entire tissue section from a tumor. (K) Abundance of CD8 T cells from tumors treated short term with IM + anti-VEGF (n = 5) or IM + anti-VEGF + αPD-L1 (n = 5), assessed by flow cytometry. (L–N) GzB (L), IFNγ (M), and TNFα (N) expression in CD8 T cells from tumors treated as in (K). (O) Assessment of the benefits of early versus late incorporation of anti-PD-L1. Ctrl (n = 5), IM + anti-VEGF (n = 7), IM + anti-VEGF + late anti-PD-L1 (n = 4), IM + anti-VEGF + early anti-PD-L1 (n = 7), anti-VEGF + anti-PD-L1 (n = 6). (P) The combination of a TCA (e.g., imipramine) and VEGF/VEGFR inhibitors induces autophagy in cancer cells and remodels the tumor vasculature, conveying survival benefit for mice bearing GBM. Imipramine reprograms M2-like TAMs to more pro-inflammatory phenotype, via inhibition of histamine receptor signaling. Consequent to the dual treatment, CD8 and CD4 T cells are recruited and activated to evoke their cytotoxic effects. The inclusion of anti-PD-L1 in the therapeutic regimen helps sustain the immune response and increases survival benefit. (Para break) Data in all quantitative panels are presented as mean ± SEM. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns, no statistical significance. Statistical analysis by Mann-Whitney test or one-way ANOVA, unless otherwise stated.

Article Snippet: InVivo MAb anti-mouse CD4 (clone GK1.5) , BioXCell , Cat #: BE0003-1; RRID: AB_1107636.

Techniques: Cell Function Assay, Immunostaining, Flow Cytometry, Expressing, Staining, Inhibition, MANN-WHITNEY

Journal: Cancer Cell

Article Title: Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity

doi: 10.1016/j.ccell.2022.08.014

Figure Lengend Snippet:

Article Snippet: InVivo MAb anti-mouse CD4 (clone GK1.5) , BioXCell , Cat #: BE0003-1; RRID: AB_1107636.

Techniques: Purification, Plasmid Preparation, Virus, Recombinant, Activation Assay, Staining, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Fluorescence, cAMP Assay, Competitive ELISA, Selection, Cell Isolation, Derivative Assay, Negative Control, shRNA, Software

Journal: OncoImmunology

Article Title: Anti-CD137 enhances anti-CD20 therapy of systemic B-cell lymphoma with altered immune homeostasis but negligible toxicity

doi: 10.1080/2162402x.2016.1192740

Figure Lengend Snippet: Figure 1. Treatment with aCD20 and aCD137 enhances survival against B lymphoma. (A) Tumor injection and treatment design. 1 £ 105 BL3750 cells were injected intra- venously at day (D) 0. Control Ig (2A3, 250 mg i.p.) or anti-CD20 (clone 5D2, 250 mg i.p.) was administered on day 4, and anti-CD137 (clone 3H3, 100 mg i.p.) either alone or in combination on day 5 in C57BL6 wild-type (WT) and various gene-targeted mice (FcRIII¡/¡, FcRIV¡/¡, and FcgR¡/¡). (B) Results are pooled from three independent experiments (n (2A3) D 24; n (5D2) D 31; n (3H3) D 12; n (5D2C3H3) D 34). Statistical analysis was calculated using Mantel–Cox test. p < 0.01 and p < 0.001 as indi- cated. (C) Results representative from one experiment, n D 6 each group. p < 0.05 and p < 0.01 as indicated. (D) Results representative from one experiment, n D 6 each group. (E) Results are pooled from two independent experiments (n (2A3) D 14; n (5D2) D 15; n (3H3) D 6; n (5D2C3H3) D 16). Statistical analysis was calculated using Mantel–Cox test. p < 0.01 as indicated. (F) Tumor inoculation (1 £ 105 BL3750 cells, i.v.) on D0 was followed by administration of anti-CD20 (clone 5D2, 250 mg i. p.) on day 4, and anti-CD137 (clone 3H3, 100 mg i.p.) at day 5, either alone or in combination in C57BL6 WT mice. Antibody treatment was then applied weekly until sacri- fice. Data shown is pooled from two independent experiments (n (2A3) D 16, n (5D2) D 6, n (3H3) D 6, n (5D2C3H3) D 16). p < 0.001, and p < 0.001 as indicated. (G) WT mice survival after single treatment with anti-CD20 (clone 5D2, 250 mg i.p. 20 d after BL3750 inoculation) and anti-CD137 (clone 3H3, 100 mg i.p. 21 d after BL3750 inoculation). Results are representative of one experiment, n D 6, each group. p < 0.01 as indicated.

Article Snippet: On day 5 post BL3750 lymphoma inoculation, anti-CD137 mAb (3H3, Bio X Cell) was administered (100 mg i.p. per injection).

Techniques: Injection, Control

Journal: OncoImmunology

Article Title: Anti-CD137 enhances anti-CD20 therapy of systemic B-cell lymphoma with altered immune homeostasis but negligible toxicity

doi: 10.1080/2162402x.2016.1192740

Figure Lengend Snippet: Figure 2. Treatment with aCD20 and aCD137 reduces blood B-cell numbers in mice bearing B-cell lymphoma. Blood samples were collected for hemogram assessment according to the experimental design shown in (A). 1 £ 105 BL3750 cells were injected i.v. at D0, and followed by treatment with control Ig (2A3, 250 mg i.p.) or anti- CD20 (clone 5D2, 250 mg i.p.) on day 4, and anti-CD137 (clone 3H3, 100 mg i.p.) either alone or in combination on day 5 in C57BL6 WT or various FcR-deficient mice, as indicated. Peripheral blood lymphocyte (B), and B cell numbers (C). Results are representative of one experiment n D 6 per group. Statistical analysis was calculated using Multiple T tests using the Holm–Sidak Method for multiple comparisons, where p < 0.05 was considered statistically significant between respective anti-CD137 (clone 3H3), anti-CD20 (clone 5D2), 5D2 C 3H3 groups versus (2A3) isotype control-treated mice.

Article Snippet: On day 5 post BL3750 lymphoma inoculation, anti-CD137 mAb (3H3, Bio X Cell) was administered (100 mg i.p. per injection).

Techniques: Injection, Control

Journal: OncoImmunology

Article Title: Anti-CD137 enhances anti-CD20 therapy of systemic B-cell lymphoma with altered immune homeostasis but negligible toxicity

doi: 10.1080/2162402x.2016.1192740

Figure Lengend Snippet: Figure 3. Treatment with aCD20 and aCD137 induces BAFF and cytokines in plasma with or without B lymphoma inoculation. Control Ig (2A3, 250 mg i.p.) or anti-CD20 (clone 5D2, 250 mg i.p.) was administered on day (D) 0, and anti-CD137 (clone 3H3, 100 mg i.p.) either alone or in combination on D1 in C57BL6 wild-type (WT) or gene- targeted mice. In some experiments (B, D), 1 £ 105 BL3750 cells were injected intravenously on day (D) 0, and control Ig (2A3, 250 mg i.p.) or anti-CD20 (clone 5D2, 250 mg i.p.) was administered on day (D) 4, and anti-CD137 (clone 3H3, 100 mg i.p.) either alone or in combination on D5 in WT or gene-targeted mice. (A) and (B) BAFF assessment from plasma of mice at D2, D5, D8 and D15 post anti-CD20 treatment. Data is representative of two experiment, n D 5 each group (n D 10 total).

Article Snippet: On day 5 post BL3750 lymphoma inoculation, anti-CD137 mAb (3H3, Bio X Cell) was administered (100 mg i.p. per injection).

Techniques: Clinical Proteomics, Control, Injection